- Title
- Epigenetically reprogrammed methylation landscape drives the DNA self-assembly and serves as a universal cancer biomarker
- Creator
- Ibn Sina, Abu Ali; Carrascosa, Laura G.; Korbie, Darren; Trau, Matt; Liang, Ziyu; Grewal, Yadveer S.; Wardiana, Andri; Shiddiky, Muhammad J. A.; Gardiner, Robert A.; Samaratunga, Hemamali; Gandhi, Maher K.; Scott, Rodney J.
- Relation
- Nature Communications Vol. 9, no. 4915
- Publisher Link
- http://dx.doi.org/10.1038/s41467-018-07214-w
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2018
- Description
- Epigenetic reprogramming in cancer genomes creates a distinct methylation landscape encompassing clustered methylation at regulatory regions separated by large intergenic tracks of hypomethylated regions. This methylation landscape that we referred to as Methylscape is displayed by most cancer types, thus may serve as a universal cancer biomarker. To-date most research has focused on the biological consequences of DNA Methylscape changes whereas its impact on DNA physicochemical properties remains unexplored. Herein, we examine the effect of levels and genomic distribution of methylcytosines on the physicochemical properties of DNA to detect the Methylscape biomarker. We find that DNA polymeric behaviour is strongly affected by differential patterning of methylcytosine, leading to fundamental differences in DNA solvation and DNA-gold affinity between cancerous and normal genomes. We exploit these Methylscape differences to develop simple, highly sensitive and selective electrochemical or colorimetric one-step assays for the detection of cancer. These assays are quick, i.e., analysis time ≤10 minutes, and require minimal sample preparation and small DNA input.
- Subject
- cancer genomes; cancer biomarkers; electrochemical detection; epigenetic reprogramming
- Identifier
- http://hdl.handle.net/1959.13/1409176
- Identifier
- uon:35958
- Identifier
- ISSN:2041-1723
- Rights
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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